期刊
NATURE MATERIALS
卷 13, 期 12, 页码 1157-1164出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMAT4092
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资金
- National Research Foundation of Korea [2013030789]
- Brain Korea 21 plus program [22A20130011095]
- Korean Health Technology R&D project through the Ministry of Health Welfare [HI13C-1938-010013]
Commercial anti-obesity drugs acting in the gastrointestinal tract or the central nervous system have been shown to have limited effcacy and severe side effects. Anti-obesity drug development is thus focusing on targeting adipocytes that store excess fat. Here, we show that an adipocyte-targeting fusion-oligopeptide gene carrier consisting of an adipocyte-targeting sequence and 9-arginine (ATS-9R) selectively transfects mature adipocytes by binding to prohibitin. Injection of ATS-9R into obese mice confirmed specific binding of ATS-9R to fat vasculature, internalization and gene expression in adipocytes. We also constructed a short-hairpin RNA (shRNA) for silencing fatty-acid-binding protein 4 (shFABP4), a key lipid chaperone in fatty-acid uptake and lipid storage in adipocytes. Treatment of obese mice with ATS-9R/shFABP4 led to metabolic recovery and body-weight reduction (>20%). The ATS-9R/shFABP4 oligopeptide complex could prove to be a safe therapeutic approach to regress and treat obesity as well as obesity-induced metabolic syndromes.
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