期刊
SLEEP AND BREATHING
卷 19, 期 3, 页码 873-882出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11325-014-1116-4
关键词
Toll-like receptor 2; Toll-like receptor 6; Obstructive sleep apnea; Intermittent hypoxia with re-oxygenation
资金
- National Science Council, Taiwan [NMRPG8B6191, NMRPG8A0241]
- Chang Gung Memorial Hospital, Taiwan [CMRPG8A0981]
Toll-like receptor (TLR) 2 can heterodimerise with TLR6 to detect diacylated lipoproteins. Hypoxia inducible factor-1 alpha co-ordinates selective induction of TLR2 and TLR6 during persistent hypoxia. We hypothesized that TLR 2/6 co-expression may be upregulated by chronic intermittent hypoxia with re-oxygenation (IHR) in obstructive sleep apnea (OSA). TLR2/6 expressions on blood immune cells were measured in 144 patients with sleep-disordered breathing (SDB), including primary snoring (PS, n = 24), moderate to severe OSA (MSO, n = 60), very severe OSA (VSO, n = 36), and very severe OSA on continuous positive airway pressure (CPAP) treatment (VSOC, n = 24). An in vitro IHR experiment was also undertaken. Patients in both the MSO and VSO groups had increased TLR2/6 co-expression on CD16(+) neutrophil than those in the PS group. Patients in the VSOC group had reduced TLR2/6 co-expression on neutrophil than those in either the MSO or VSO group. Blood absolute neutrophil count was positively but weakly correlated with TLR2/6 co-expression on neutrophil. TLR2/6 co-expressions on both CD14(+) monocyte and CD3(+)CD4(+)T helper cell, and TLR2 expressions on both monocyte and T helper cell in SDB patients with low Minimum SaO(2) (a parts per thousand broken vertical bar 70 %) were all higher than those with high Minimum SaO(2). In vitro IHR for 1-4 days resulted in TLR2/6 co-upregulation on both neutrophil and monocyte. OSA patients had increased TLR2/6 co-expressions on blood immune cells, which were related to their immune cell counts and could be reversed with CPAP treatment. In vitro IHR could induce TLR2/6 co-upregulation.
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