期刊
NATURE MATERIALS
卷 10, 期 10, 页码 799-806出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMAT3101
关键词
-
类别
资金
- Natural Sciences and Engineering Research Council (NSERC)
- Canadian Institutes of Health Research (CIHR) [MOP-6279]
- Le Fonds Quebecoise de la recherche sur la nature et les technologies
- Vision Science Research Program
Three-dimensional (3D) protein-patterned scaffolds provide a more biomimetic environment for cell culture than traditional two-dimensional surfaces, but simultaneous 3D protein patterning has proved difficult. We developed a method to spatially control the immobilization of different growth factors in distinct volumes in 3D hydrogels, and to specifically guide differentiation of stem/progenitor cells therein. Stem-cell differentiation factors sonic hedgehog (SHH) and ciliary neurotrophic factor (CNTF) were simultaneously immobilized using orthogonal physical binding pairs, barnase-barstar and streptavidin-biotin, respectively. Barnase and streptavidin were sequentially immobilized using two-photon chemistry for subsequent concurrent complexation with fusion proteins barstar-SHH and biotin-CNTF, resulting in bioactive 3D patterned hydrogels. The technique should be broadly applicable to the patterning of a wide range of proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据