期刊
NATURE MATERIALS
卷 8, 期 5, 页码 432-437出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMAT2419
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资金
- NYSTAR Faculty Development Program Award
- NYSTAR CAT
- US National Science Foundation's CAREER award [0547330]
- USDA NRI
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0547330] Funding Source: National Science Foundation
Proteins are important biomaterials and are generally produced in living cells. Here, we show a novel DNA hydrogel that is capable of producing functional proteins without any living cells. This protein-producing gel (termed 'the P-gel system' or 'P-gel') consists of genes as part of the gel scaffolding. This is the first time that a hydrogel has been used to produce proteins. The efficiency was about 300 times higher than current, solution-based systems. In terms of volumetric yield, the P-gel produced up to 5 mg ml(-1) of functional proteins. The mechanisms behind the high efficiency and yield include improved gene stability, higher local concentration and a faster enzyme turnover rate due to a closer proximity of genes. We have tested a total of 16 different P-gels and have successfully produced all 16 proteins including membrane and toxic proteins, demonstrating that the P-gel system can serve as a general protein production technology.
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