期刊
NATURE GENETICS
卷 43, 期 9, 页码 854-U60出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ng.905
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资金
- US National Institutes of Health (NIH) [1DP1OD003936]
- NIH-National Cancer Institute (NCI) Physical Sciences Oncology Center at the Massachusetts Institute of Technology [U54CA143874]
- NIH [R01-CA133404, R01-GM34277]
- NCI [PO1-CA42063, P30-CA14051]
- Howard Hughes Medical Institute
- Paul and Cleo Schimmel Scholarship
- Natural Sciences and Engineering Research Council of Canada
MicroRNAs (miRNAs) are short, highly conserved noncoding RNA molecules that repress gene expression in a sequence-dependent manner. We performed single-cell measurements using quantitative fluorescence microscopy and flow cytometry to monitor a target gene's protein expression in the presence and absence of regulation by miRNA. We find that although the average level of repression is modest, in agreement with previous population-based measurements, the repression among individual cells varies dramatically. In particular, we show that regulation by miRNAs establishes a threshold level of target mRNA below which protein production is highly repressed. Near this threshold, protein expression responds sensitively to target mRNA input, consistent with a mathematical model of molecular titration. These results show that miRNAs can act both as a switch and as a fine-tuner of gene expression.
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