期刊
NATURE CHEMISTRY
卷 5, 期 2, 页码 132-139出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEM.1546
关键词
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资金
- Swiss National Science Foundation
- Chemical National Centre of Competence in Research Biology, European Research Council [243016]
- Ecole Polytechnique Federale de Lausanne
- Federation of European Biochemical Societies long-term fellowship
- Fonds der Chemischen Industrie
- Emmy Noether program of the Deutsche Forschungsgemeinschaft
- Deutsche Forschungsgemeinschaft [SPP 1623]
- [TRR83]
- European Research Council (ERC) [243016] Funding Source: European Research Council (ERC)
- MRC [MR/K01577X/1, MC_UU_12010/9] Funding Source: UKRI
- Medical Research Council [MC_UU_12010/9, MR/K01577X/1] Funding Source: researchfish
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.
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