期刊
NATURE CHEMICAL BIOLOGY
卷 10, 期 8, 页码 623-U152出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.1550
关键词
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Identification and validation of drug-resistant mutations can provide important insights into the mechanism of action of a compound. Here we demonstrate the feasibility of such an approach in mammalian cells using next-generation sequencing of drug-resistant clones and CRISPR-Cas9-mediated gene editing on two drug-target pairs, 6-thioguanine-HPRT1 and triptolide-ERCC3. We showed that disrupting functional HPRT1 allele or introducing ERCC3 point mutations by gene editing can confer drug resistance in cells.
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