期刊
NATURE CHEMICAL BIOLOGY
卷 10, 期 12, 页码 1003-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.1656
关键词
-
资金
- R. Meng and the Proteomic Mass Spectrometry Core of the National Facilities for Protein Sciences
- National Basic Research Program of China [2010CB912300, 2012CB917301]
- National Natural Science Foundation of China [21225206, 91313301]
- Peking University Principal Foundation
Small molecules that specifically activate an intracellular protein of interest are highly desirable. A generally applicable strategy, however, remains elusive. Herein we describe a small molecule-triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eliminating a chemically caged protein side chain within living cells. This method permits the efficient activation of a given protein (for example, an enzyme) in its native cellular context within minutes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据