期刊
NATURE CHEMICAL BIOLOGY
卷 8, 期 5, 页码 434-436出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.921
关键词
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资金
- National Science Foundation [CBET-0449080]
- New York State Office of Science
- National Institutes of Health [R43 GM087766, R43 GM086965]
- National Institutes of Health National Center for Research Resources [1 P41 RR018502-01]
- LASPAU
- Universidad Antonio Narino
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1159581] Funding Source: National Science Foundation
We performed bottom-up engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. The glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, we successfully transferred glycans to eukaryotic proteins.
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