期刊
NATURE CHEMICAL BIOLOGY
卷 6, 期 10, 页码 774-781出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.424
关键词
-
资金
- Division of Intramural Research of the US National Institutes of Health, National Institute of Environmental Health Sciences
- Swedish Foundation for Strategic Research
- Swedish Research Council
- Swedish Cancer Society
- Umea University
Maintaining the chemical identity of DNA depends on ribonucleotide exclusion by DNA polymerases. However, ribonucleotide exclusion during DNA synthesis in vitro is imperfect. To determine whether ribonucleotides are incorporated during DNA replication in vivo, we substituted leucine or glycine for an active-site methionine in yeast DNA polymerase epsilon ( Pol epsilon). Ribonucleotide incorporation in vitro was three-fold lower for M644L and 11-fold higher for M644G Pol epsilon compared to wildtype Pol epsilon. This hierarchy was recapitulated in vivo in yeast strains lacking RNase H2. Moreover, the pol2-M644G rnh201 Delta strain progressed more slowly through S phase, had elevated dNTP pools and generated 2-5-base-pair deletions in repetitive sequences at a high rate and in a gene orientation-dependent manner. The data indicate that ribonucleotides are incorporated during replication in vivo, that they are removed by RNase H2-dependent repair and that defective repair results in replicative stress and genome instability via DNA strand misalignment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据