期刊
NATURE CHEMICAL BIOLOGY
卷 6, 期 4, 页码 255-257出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.321
关键词
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资金
- US National Institutes of Health [GM53395]
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan [20021008, 19680020]
- Precursory Research for Embryonic Science and Technology (PRESTO)
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [19680020, 20021008] Funding Source: KAKEN
We have synthesized a photosensitive ( or caged) 4-carboxymethoxy-5,7-dinitroindolinyl (CDNI) derivative of gamma-aminobutyric acid (GABA). Two-photon excitation of CDNI-GABA produced rapid activation of GABAergic currents in neurons in brain slices with an axial resolution of approximately 2 mu m and enabled high-resolution functional mapping of GABA-A receptors. Two-photon uncaging of GABA, the main inhibitory neurotransmitter, should allow detailed studies of receptor function and synaptic integration with subcellular precision.
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