期刊
NATURE CHEMICAL BIOLOGY
卷 4, 期 8, 页码 440-443出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio0808-440
关键词
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资金
- NIGMS NIH HHS [R01 GM077248-02, R01 GM077248] Funding Source: Medline
The development of single-molecule tools has significantly impacted the way we think about biochemical processes. Watching a single protein in action allows us to observe kinetic details and rare subpopulations that are hidden in ensemble-averaging techniques. I will discuss here the pros and cons of the single-molecule approach in studying ligand binding in macromolecular systems and how these techniques can be applied to characterize the behavior of large multicomponent biochemical systems.
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