期刊
NATURE CELL BIOLOGY
卷 15, 期 3, 页码 317-324出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb2681
关键词
-
类别
资金
- Canadian Institute for Health Research (CIHR) [MOP-114899]
- Fonds de Recherche Quebec Sante (FRQS)
- Atip-Avenir programme
- Centre National de la Recherche Scientifique (CNRS)
- Canadian Center of Excellence in Commercialization and Research (CECR)
- Canada Foundation for Innovation (CFI)
- Fonds de Recherche du Quebec en Sante (FRQS)
- [R01GM46425]
- [R01GM73164]
Collective cell movements contribute to development and metastasis. The small GTPase Rac is a key regulator of actin dynamics and cell migration but the mechanisms that restrict Rac activation and localization in a group of collectively migrating cells are unknown. Here, we demonstrate that the small GTPases Rab5 and Rab11 regulate Rac activity and polarization during collective cell migration. We use photoactivatable forms of Rac to demonstrate that Rab11 acts on the entire group to ensure that Rac activity is properly restricted to the leading cell through regulation of cell-cell communication. In addition, we show that Rab11 binds to the actin cytoskeleton regulator Moesin and regulates its activation in vivo during migration. Accordingly, reducing the level of Moesin activity also affects cell-cell communication, whereas expressing active Moesin rescues loss of Rab11 function. Our model suggests that Rab11 controls the sensing of the relative levels of Rac activity in a group of cells, leading to the organization of individual cells in a coherent multicellular motile structure.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据