期刊
NATURE CELL BIOLOGY
卷 15, 期 3, 页码 241-248出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb2671
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资金
- Spanish MICINN MIMECO [BFU2009-07975/BMC, BFU2012-32522, CENIT ONCOLOGICA-20091016, Consolider-Ingenio C3D CSD2006-23]
- CDTI
- Generalitat de Catalunya [SGR Agaur 2009 CG041413]
- ICREA Funding Source: Custom
During interphase in Drosophila neuroblasts, the Centrobin (CNB)-positive daughter centriole retains pericentriolar material (PCM) and organizes an aster that is a key determinant of the orientation of cell division. Here we show that daughter centrioles depleted of CNB cannot fulfil this function whereas mother centrioles that carry ectopic CNB can. CNB co-precipitates with a set of centrosomal proteins that include gamma-TUB, ANA2, CNN, SAS-4, ASL, DGRIP71, POLO and SAS-6. Following chemical inhibition of POLO or removal of three POLO phosphorylation sites present in CNB, the interphase microtubule aster is lost. These results demonstrate that centriolar CNB localization is both necessary and sufficient to enable centrioles to retain PCM and organize the interphase aster in Drosophila neuroblasts. They also reveal an interphase function for POLO in this process that seems to have co-opted part of the protein network involved in mitotic centrosome maturation.
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