期刊
NATURE CELL BIOLOGY
卷 14, 期 4, 页码 416-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb2446
关键词
-
类别
资金
- UK Medical Research Council [U105178790]
- Lister Institute prize fellowship
- MRC [MC_U105178790] Funding Source: UKRI
- Medical Research Council [MC_U105178790] Funding Source: researchfish
Subcellular localization of mRNAs by cytoskeletal motors plays critical roles in the spatial control of protein function(1). However, optical limitations of studying mRNA transport in vivo mean that there is little mechanistic insight into how transcripts are packaged and linked to motors, and how the movement of mRNA motor complexes on the cytoskeleton is orchestrated. Here, we have reconstituted transport of mRNPs containing specific RNAs in vitro. We show directly that mRNAs that are either apically localized or non-localized in Drosophila melanogaster embryos associate with the dynein motor and move bidirectionally on individual microtubules, with localizing mRNPs exhibiting a strong minus-end-directed bias. Single-molecule fluorescence measurements reveal that RNA localization signals increase the average number of dynein and dynactin components recruited to individual mRNPs. We find that, surprisingly, individual RNA molecules are present in motile mRNPs in vitro and provide evidence that this is also the case in vivo. Thus, RNA oligomerization is not obligatory for transport. Our findings lead to a model in which RNA localization signals produce highly polarized distributions of transcript populations through modest changes in motor copy number on single mRNA molecules.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据