期刊
NATURE CELL BIOLOGY
卷 14, 期 8, 页码 865-U197出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb2527
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- National Institute of General Medical Sciences [R01GM098394]
Regulated centrosome biogenesis is required for accurate cell division and for maintaining genome integrity(1). Centrosomes consist of a centriole pair surrounded by protein network known as pericentriolar material(1) (PCM). PCM assembly is a tightly regulated, critical step that determines the size and capability of centrosomes(2-4). Here, we report a role for tubulin in regulating PCM recruitment through the conserved centrosomal protein Sas-4. Tubulin directly binds to Sas-4; together they are components of cytoplasmic complexes of centrosomal proteins(5,6). A Sas-4 mutant, which cannot bind tubulin, enhances centrosomal protein complex formation and has abnormally large centrosomes with excessive activity. These results suggest that tubulin negatively regulates PCM recruitment. Whereas tubulin-GTP prevents Sas-4 from forming protein complexes, tubulin-GDP promotes it. Thus, the regulation of PCM recruitment by tubulin depends on its GTP/GDP-bound state. These results identify a role for tubulin in regulating PCM recruitment independent of its well-known role as a building block of microtubules(7). On the basis of its guanine-bound state, tubulin can act as a molecular switch in PCM recruitment.
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