期刊
NATURE CELL BIOLOGY
卷 13, 期 4, 页码 453-U242出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb2204
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资金
- Eli Lilly Japan
- British Council Japan Association
- MRC
- Heiser Foundation
- NIH [AI069345, AI045148, AI042999]
- Wellcome Trust
- EU
- Medical Research Council [G0600194] Funding Source: researchfish
- MRC [G0600194] Funding Source: UKRI
mTOR (mammalian target of rapamycin) signalling and macroautophagy (henceforth autophagy) regulate numerous pathological and physiological processes, including cellular responses to altered nutrient levels. However, the mechanisms regulating mTOR and autophagy remain incompletely understood. Lysosomes are dynamic intracellular organelles(1,2) intimately involved both in the activation of mTOR complex 1 (mTORC1) signalling and in degrading autophagic substrates(3-8). Here we report that lysosomal positioning coordinates anabolic and catabolic responses with changes in nutrient availability by orchestrating early plasma-membrane signalling events, mTORC1 signalling and autophagy. Activation of mTORC1 by nutrients correlates with its presence on peripheral lysosomes that are physically close to the upstream signalling modules, whereas starvation causes perinuclear clustering of lysosomes, driven by changes in intracellular pH. Lysosomal positioning regulates mTORC1 signalling, which in turn influences autophagosome formation. Lysosome positioning also influences autophagosome-lysosome fusion rates, and thus controls autophagic flux by acting at both the initiation and termination stages of the process. Our findings provide a physiological role for the dynamic state of lysosomal positioning in cells as a coordinator of mTORC1 signalling with autophagic flux.
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