期刊
NATURE CELL BIOLOGY
卷 11, 期 12, 页码 1433-U102出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1991
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
Autophagy is a bulk degradation process in eukaryotic cells and has fundamental roles in cellular homeostasis. The origin and source of autophagosomal membranes are long-standing questions in the field. Using electron microscopy, we show that, in mammalian culture cells, the endoplasmic reticulum ( ER) associates with early autophagic structures called isolation membranes (IMs). Overexpression of an Atg4B mutant, which causes defects in autophagosome formation, induces the accumulation of ER-IM complexes. Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.
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