期刊
NATURE CELL BIOLOGY
卷 12, 期 1, 页码 87-U234出版社
NATURE PORTFOLIO
DOI: 10.1038/ncb2009
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资金
- NSF [MCB0918220]
- NIH [GM060396]
- DOE [DE-FG02-03ER15449]
- Swedish Research Council Formas
- Deutsche Forschungsgemeinschaft
- EMBO
- King Abdullah University of Science and Technology (KAUST) [KUS-F1-021-31]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM060396] Funding Source: NIH RePORTER
The continuing rise in atmospheric CO2 causes stomatal pores in leaves to close and thus globally affects CO2 influx into plants, water use efficiency and leaf heat stress(1-4). However, the CO2-binding proteins that control this response remain unknown. Moreover, which cell type responds to CO2, mesophyll or guard cells, and whether photosynthesis mediates this response are matters of debate(5-8). We demonstrate that Arabidopsis thaliana double-mutant plants in the beta-carbonic anhydrases beta CA1 and beta CA4 show impaired CO2-regulation of stomatal movements and increased stomatal density, but retain functional abscisic-acid and blue-light responses. beta CA-mediated CO2-triggered stomatal movements are not, in first-order, linked to whole leaf photosynthesis and can function in guard cells. Furthermore, guard cell beta CA-overexpressing plants exhibit instantaneous enhanced water use efficiency. Guard cell expression of mammalian alpha CAII complements the reduced sensitivity of ca1 ca4 plants, showing that carbonic anhydrase-mediated catalysis is an important mechanism for beta CA-mediated CO2 induced stomatal closure and patch clamp analyses indicate that CO2/HCO3-transfers the signal to anion channel regulation. These findings, together with ht1-2 (ref.9) epistasis analysis demonstrate that carbonic anhydrases function early in the CO2 signalling pathway, which controls gas-exchange between plants and the atmosphere.
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