期刊
NATURE CELL BIOLOGY
卷 11, 期 11, 页码 1363-U241出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1983
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资金
- Newton Trust
- Canadian Institute of Health Research
- FEBS
- Churchill Foundation Scholarship
- UK Medical Research Council (MRC)
- Wellcome Trust
- Cancer Research UK
- Medical Research Council [G0600332, MC_U105359877, G9900064, G0700651] Funding Source: researchfish
- MRC [MC_U105359877, G9900064, G0600332, G0700651] Funding Source: UKRI
The anaphase-promoting complex (APC/C), a ubiquitin ligase, is the target of the spindle-assembly checkpoint (SAC), and it ubiquitylates protein substrates whose degradation regulates progress through mitosis(1-3). The identity of the ubiquitin-conjugating (E2) enzymes that work with the APC/C is unclear. In an RNA interference (RNAi) screen for factors that modify release from drug-induced SAC activation, we identified the E2 enzyme UBE2S as an APC/C auxiliary factor that promotes mitotic exit. UBE2S is dispensable in a normal mitosis, but its depletion prolongs drug-induced mitotic arrest and suppresses mitotic slippage. In vitro, UBE2S elongates ubiquitin chains initiated by the E2 enzymes UBCH10 and UBCH5, enhancing the degradation of APC/C substrates by the proteasome. Indeed, following release from SAC-induced mitotic arrest, UBE2S-depleted cells neither degrade crucial APC/C substrates, nor silence this checkpoint, whereas bypassing the SAC through BUBR1 depletion or Aurora-B inhibition negates the requirement for UBE2S. Thus, UBE2S functions with the APC/C in a two-step mechanism to control substrate ubiquitylation that is essential for mitotic exit after prolonged SAC activation, providing a new model for APC/C function in human cells.
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