期刊
NATURE BIOTECHNOLOGY
卷 31, 期 3, 页码 227-229出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2501
关键词
-
资金
- US National Institutes of Health (NIH) [DP1 GM105378]
- NIH [R01 GM088040, P50 HG005550, K01 AG031300]
- Jim and Ann Orr Research Scholar Award
- Massachusetts General Hospital
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR) CRISPR-associated (Gas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator like effector nucleases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据