期刊
NATURE BIOTECHNOLOGY
卷 31, 期 3, 页码 230-232出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2507
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资金
- National Research Foundation of Korea [2012-0001225]
- ToolGen, Inc.
- National Research Foundation of Korea [2010-0018267] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
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