期刊
NATURE BIOTECHNOLOGY
卷 29, 期 12, 页码 1132-U113出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.2051
关键词
-
资金
- International Stem Cell Forum
- Ministry of Science and Technology of China [2006AA02A102]
- Japan Society for the Promotion of Science (JSPS)
- MEXT
- Swiss National Science Foundation [4046-114410]
- North West Science Fund, UK
- One North East Regional Developmental Agency
- Medical Research Council, UK
- Newcastle University
- Australian Stem Cell Centre
- Netherlands Proteomics Consortium [T4-3]
- Stem Cell Network, Canada
- MEXT, Japan
- Singapore Stem Cell Consortium (SSCC)
- Agency for Science Technology and Research (AstarSTAR)
- Genome Institute of Singapore Core Genotyping Lab
- Academy of Finland [218050]
- Sigrid Juselius Foundation
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Departamento de Ciencia e Tecnologia do Ministerio da Saude (CNPq/MS/DECIT)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Judy
- Sidney Swartz
- Faculty of Medicine
- University of New South Wales (UNSW)
- National Health and Medical Research Council (NHMRC) [568969]
- South Eastern Sydney and Illawarra Area Health Service (SEIAHS)
- Tampere University Hospital [9F217]
- Grants-in-Aid for Scientific Research [20114007, 19002014] Funding Source: KAKEN
- Biotechnology and Biological Sciences Research Council [BB/E006159/1] Funding Source: researchfish
- British Heart Foundation [PG/09/027/27141] Funding Source: researchfish
- Medical Research Council [G0301182, MC_qA137914, G0801059, G0700092, G1001045, G0801098, G0700785, G113/30, MC_qA137863, G0801057, MC_qA137916, G0300484] Funding Source: researchfish
- BBSRC [BB/E006159/1] Funding Source: UKRI
- MRC [MC_qA137916, G0300484, G0801059, G113/30, MC_qA137914, G0700092, G0700785, G1001045, G0801098, G0301182, G0801057] Funding Source: UKRI
The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据