期刊
NATURE BIOTECHNOLOGY
卷 29, 期 1, 页码 68-72出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1732
关键词
-
资金
- US National Institutes of Health [GM071440, NS051630/MH076090/MH078972]
- University of Chicago
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM071440] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH078972, R01MH076090, R56MH076090] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R21NS067461, R01NS051630] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P50AG025688] Funding Source: NIH RePORTER
In contrast to 5-methylcytosine (5-mC), which has been studied extensively(1-3), little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types(4,5). Here we present a method for determining the genome-wide distribution of 5-hmC. We use the T4 bacteriophage beta-glucosyltransferase to transfer an engineered glucose moiety containing an azide group onto the hydroxyl group of 5-hmC. The azide group can be chemically modified with biotin for detection, affinity enrichment and sequencing of 5-hmC-containing DNA fragments in mammalian genomes. Using this method, we demonstrate that 5-hmC is present in human cell lines beyond those previously recognized(4). We also find a gene expression level-dependent enrichment of intragenic 5-hmC in mouse cerebellum and an age-dependent acquisition of this modification in specific gene bodies linked to neurodegenerative disorders.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据