期刊
NATURE BIOTECHNOLOGY
卷 28, 期 6, 页码 611-U102出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1620
关键词
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资金
- Knut and Alice Wallenberg Foundation
- Novo Nordisk Foundation
- Soderberg's Foundation
- Swedish Research Council
- Swedish Cancer Foundation
- Harvard Stem Cell Institute
- Leducq Foundation
We describe a system for culturing human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche. The system is devoid of animal products and feeder cells and contains only one undefined component, human albumin. The hES cells self-renewed with normal karyotype for at least 4 months (20 passages), after which the cells could produce teratomas containing cell lineages of all three germ layers. When plated on laminin-511 in small clumps, hES cells spread out in a monolayer, maintaining cellular homogeneity with approximately 97% OCT4-positive cells. Adhesion of hES cells was dependent on alpha 6 beta 1 integrin. The use of homogeneous monolayer hES or iPS cell cultures provides more controllable conditions for the design of differentiation methods. This xeno-free and feeder-free system may be useful for the development of cell lineages for therapeutic purposes.
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