期刊
NATURE BIOTECHNOLOGY
卷 28, 期 3, 页码 256-U111出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.1608
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [17025044]
- Ministry of Health, Welfare and Labor, Japan
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [17025044] Funding Source: KAKEN
Huntington's Disease (HD) is a dominantly inherited pathology caused by the accumulation of mutant huntingtin protein (HTT) containing an expanded polyglutamine (polyQ) tract. As the polyglutamine binding peptide 1 (QBP1) is known to bind an expanded polyQ tract but not the polyQ motif found in normal HTT, we selectively targeted mutant HTT for degradation by expressing a fusion molecule comprising two copies of QBP1 and copies of two different heat shock cognate protein 70 (HSC70)-binding motifs in cellular and mouse models of HD. Chaperone-mediated autophagy contributed to the specific degradation of mutant HTT in cultured cells expressing the construct. Intrastriatal delivery of a virus expressing the fusion molecule ameliorated the disease phenotype in the R6/2 mouse model of HD. Similar adaptor molecules comprising HSC70-binding motifs fused to an appropriate structure-specific binding agent(s) may have therapeutic potential for treating diseases caused by misfolded proteins other than those with expanded polyQ tracts.
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