期刊
NATURE
卷 516, 期 7531, 页码 405-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nature13804
关键词
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资金
- University of Bath
- DFG [SCHU1014/8-1]
- LOEWE Center for Cell and Gene Therapy Frankfurt/Hessian Ministry of Higher Education, Research and the Arts [III L 4-518/17.004]
- [ERC-2011-AdG 294742]
Naive embryonic stem cells hold great promise for research and therapeutics as they have broad and robust developmental potential. While such cells are readily derived from mouse blastocysts it has not been possible to isolate human equivalents easily(1,2), although human naive-like cells have been artificially generated (rather than extracted) by coercion of human primed embryonic stem cells by modifying culture conditions(2-4) or through transgenic modification(5). Here we show that a sub-population within cultures of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) manifests key properties of naive state cells. These naive-like cells can be genetically tagged, and are associated with elevated transcription of HERVH, a primate-specific endogenous retrovirus. HERVH elements provide functional binding sites for a combination of naive pluripotency transcription factors, including LBP9, recently recognized as relevant to naivety inmice(6). LBP9-HERVH drives hESC-specific alternative and chimaeric transcripts, including pluripotency-modulating long non-coding RNAs. Disruption of LBP9, HERVH and HERVH-derived transcripts compromises self-renewal. These observations define HERVH expression as a hallmark of naive-like hESCs, and establish novel primate-specific transcriptional circuitry regulating pluripotency.
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