期刊
NATURE
卷 485, 期 7396, 页码 99-103出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nature10997
关键词
-
资金
- Medical Research Council UK [G03000341]
- Wellcome Trust [082217]
- Royal Society [RG080398]
- British Retinitis Pigmentosa Society [GR566]
- Miller's Trust
- Department of Health's National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital
- Alcon Research Institute
- Great Ormond Street Hospital Children's Charity
- US National Institutes of Health [EY06837]
- Antonio Champalimaud Vision Award (Portugal)
- Research to Prevent Blindness [EY11307, EY016805]
- European Research Council
- Fight for Sight [1779/80, 1351/52] Funding Source: researchfish
- Great Ormond Street Hospital Childrens Charity [V1221, V1257] Funding Source: researchfish
- Medical Research Council [G0700438, MR/J004553/1, G0901550, G0800791] Funding Source: researchfish
- National Institute for Health Research [NIHR-RP-011-003, NF-SI-0508-10130] Funding Source: researchfish
- MRC [G0901550, MR/J004553/1, G0700438, G0800791] Funding Source: UKRI
Cell transplantation is a potential strategy for treating blindness caused by the loss of photoreceptors. Although transplanted rod-precursor cells are able to migrate into the adult retina and differentiate to acquire the specialized morphological features of mature photoreceptor cells(1), the fundamental question remains whether transplantation of photoreceptor cells can actually improve vision. Here we provide evidence of functional rod-mediated vision after photoreceptor transplantation in adult Gnat1(-/-) mice, which lack rod function and are a model of congenital stationary night blindness(2). We show that transplanted rod precursors form classic triad synaptic connections with second-order bipolar and horizontal cells in the recipient retina. The newly integrated photoreceptor cells are light-responsive with dim-flash kinetics similar to adult wild-type photoreceptors. By using intrinsic imaging under scotopic conditions we demonstrate that visual signals generated by transplanted rods are projected to higher visual areas, including V1. Moreover, these cells are capable of driving optokinetic head tracking and visually guided behaviour in the Gnat1(-/-) mouse under scotopic conditions. Together, these results demonstrate the feasibility of photoreceptor transplantation as a therapeutic strategy for restoring vision after retinal degeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据