期刊
NATURE
卷 486, 期 7404, 页码 496-501出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nature11142
关键词
-
资金
- National Institutes of Health [T32GM00806, R01GM058617, R01GM075313, U54CA143869]
The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.
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