期刊
NATURE
卷 479, 期 7371, 页码 135-U175出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nature10501
关键词
-
资金
- NHMRC
- Spanish Ministry of Science
- Spanish Ministry of Science and Innovation [BFU2008-01919, CSD2007-00015]
- NIH [R01 GM076396]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1025830] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0963400, 923128] Funding Source: National Science Foundation
- Grants-in-Aid for Scientific Research [22115501] Funding Source: KAKEN
Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification(1) during the DNA replication phase of the cell cycle(2). Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes(3) that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据