期刊
NATURE
卷 466, 期 7302, 页码 138-U161出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nature09140
关键词
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资金
- American Italian Cancer Foundation
- March of Dimes [1-FY08-372]
- National Institutes of Health [R01-GM057587, R37-CA076584, R21-AG032560]
- Stowers Institute for Medical Research
Generally, F-box proteins are the substrate recognition subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase complexes, which mediate the timely proteolysis of important eukaryotic regulatory proteins(1,2). Mammalian genomes encode roughly 70 F-box proteins, but only a handful have established functions(3,4). The F-box protein family obtained its name from Cyclin F (also called Fbxo1), in which the F-box motif (the similar to 40-amino-acid domain required for binding to Skp1) was first described(5). Cyclin F, which is encoded by an essential gene, also contains a cyclin box domain, but in contrast to most cyclins, it does not bind or activate any cyclin-dependent kinases (CDKs)(5-7). However, like other cyclins, Cyclin F oscillates during the cell cycle, with protein levels peaking in G2. Despite its essential nature and status as the founding member of the F-box protein family, Cyclin F remains an orphan protein, whose functions are unknown. Starting from an unbiased screen, we identified CP110, a protein that is essential for centrosome duplication, as an interactor and substrate of Cyclin F. Using a mode of substrate binding distinct from other F-box protein-substrate pairs, CP110 and Cyclin F physically associate on the centrioles during the G2 phase of the cell cycle, and CP110 is ubiquitylated by the SCFCyclin F ubiquitin ligase complex, leading to its degradation. siRNA-mediated depletion of Cyclin F in G2 induces centrosomal and mitotic abnormalities, such as multipolar spindles and asymmetric, bipolar spindles with lagging chromosomes. These phenotypes were reverted by co-silencing CP110 and were recapitulated by expressing a stable mutant of CP110 that cannot bind Cyclin F. Finally, expression of a stable CP110 mutant in cultured cells also promotes the formation of micronuclei, a hallmark of chromosome instability. We propose that SCFCyclin F-mediated degradation of CP110 is required for the fidelity of mitosis and genome integrity.
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