Primula latifolia Lapeyr. and Primula vulgaris Hudson flavonoids

Three flavonoids were isolated from the leaf MeOH extracts of Primula latifolia Lapeyr. and Primula vulgaris Hudson collected from Italian Alps: rutin (1) and kaempferol 3-neohesperidoside (2) from P. latifolia, and kaempferol 3-beta-O-glucopyranosyl-(1 -> 2) gentiobioside (3) from P. vulgaris. The structures were assigned on the basis of their H-1 and C-13 NMR data, including those derived from 2D NMR, as well as on HPLC-MS results. This article is the first to report on P. vulgaris tissue flavonoids after Harborne's study in 1968 and the first work ever on these compounds from P. latifolia.