期刊
NATURAL PRODUCT RESEARCH
卷 26, 期 17, 页码 1621-1625出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2011.576394
关键词
Verbascum thapsus L.; antiviral activity; pseudorabies virus (strain RC/79); methanolic extract
资金
- CONICET
- MinCyT of Cordoba
- Universidad Nacional de Rio Cuarto
- PICTOR program [BID 1728/OC-AR]
The methanolic extract of Verbascum thapsus was evaluated for its antiviral activity against the pseudorabies virus strain RC/79 (PrV), and also for its cytotoxic activity on Vero cells. The extract showed CC50 values of 1100 mu g mL(-1) and 1426 mu g mL(-1) by NRU and MTT assays, respectively. The 50% inhibitory concentration of the extract for PrV plaque formation was determined at 35 mu g mL(-1), and selectivity indices were 31.4 (NRU) and 40.7 (MTT). When cells were pre-treated with the extract prior to virus infection, the inhibition in plaque formation was 70%. PrV was highly inhibited when it was incubated with plant extract or when the extract was added during the adsorption phase (99%). However, no inhibitory effect was observed when the extract was added to the cells after the adsorption period. Thus, these results suggest that the methanolic extract of Verbascum thapsus may contain bioactive compound(s) that affect PrV mostly in the adsorption phase.
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