期刊
NANOTOXICOLOGY
卷 8, 期 2, 页码 202-211出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2013.773465
关键词
corona; hemolysis; polymeric nanoparticles; surface functionalization; toxicity; zeta potential
资金
- Medical Research Council of the United Kingdom [MRC G0701323, 13182KFDA606]
- Novartis Foundation
- Natural Environment Research Council [NE/E007791/1]
- Colt Foundation
- Medical Research Council [G0701323, G0901697] Funding Source: researchfish
- Natural Environment Research Council [NE/E007791/1] Funding Source: researchfish
- MRC [G0901697, G0701323] Funding Source: UKRI
- NERC [NE/E007791/1] Funding Source: UKRI
Nano materials are commonly functionalized to boost their physicochemical properties. However, there is little known about the impact of these modifications on cellular systems. Herein, we synthesized eight types of polymeric nanoparticles (NPs) bearing different functional groups, and investigated their effects on interactions with cellular membranes. As models for particle membrane interactions, hemolysis assays using human red blood cells and culture with A549 cells were utilized. Under protein-free conditions, the NPs showed a wide distribution of zeta potentials (zPs) which showed a good correlation with their hemolytic potential. However, in the presence of serum or lung lining fluid, the zPs of all NPs coalesced towards a single common negative value and showed neither hemolytic activity nor cytotoxicity to A549 cells. Lipase and protease treatment of the coronated particles did not restore their reactivity. These result simply proves that particle functionalization influences the stability of the particle corona which, if intact, prevents hemolytic activity and membrane disrupture.
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