期刊
NANOTOXICOLOGY
卷 7, 期 8, 页码 1373-1385出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2012.741725
关键词
nano-object(s); nano-bacterial interactions; mutagenicity; Ames test; mammalian cells
资金
- European Respiratory Society [LTRF-MC1572-2010]
- Swiss National Science Foundation [320030_138365]
- German Research Foundation [DFG SPP 1313]
- Adolphe Merkle Foundation
- Swiss National Science Foundation (SNF) [320030_138365] Funding Source: Swiss National Science Foundation (SNF)
The aim of this study was to assess the interaction of a series of well characterised nano-objects with the Gram negative bacterium Salmonella typhimurium, and how such an interaction may relate to the potential mutagenicity of nano-objects. Transmission electron microscopy showed that nanoobjects (Au-PMA-ATTO NPs, CeO2 NPs, SWCNTs and MWCNTs), as well as CAFs entered S. typhimurium. Only DEPs did not penetrate/enter the bacteria, however, were the only particle stimulus to induce any significant mutagenicity through the Ames test. Comparison with a sophisticated 3D in vitro cell model showed CAFs, DEPs, SWCNTs and MWCNTs to cause a significant increase in mammalian cell proliferation, whilst both the Au-PMA-ATTO NPs and CeO2 NPs had not significant adverse effects. In conclusion, these results indicate that various of different nano-objects are able to penetrate the double-lipid bilayer of Gram negative bacteria, although the Ames test may not be a good indicator for nano-object mutagenicity.
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