期刊
NANOTOXICOLOGY
卷 8, 期 1, 页码 88-99出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2012.752051
关键词
Balb/3T3; uptake; intracellular distribution; dissolution; radiolabelling
资金
- Unit of Immunotoxicology and Allergy of Ce.S.I.
- ECVAM Unit (IHCP, JRC-Ispra)
- Fondi Ateneo per la Ricerca from the University of Insubria
- Associazione Italiana Ricerca sul Cancro (AIRC) [IG 9168]
The mechanistic understanding of nanotoxicity requires the physico-chemical characterisation of nanoparticles (NP), and their comparative investigation relative to the corresponding ions and microparticles (MP). Following this approach, the authors studied the dissolution, interaction with medium components, bioavailability in culture medium, uptake and intracellular distribution of radiolabelled Co forms (CoNP, CoMP and Co2+) in Balb/3T3 mouse fibroblasts. Co2+ first saturates the binding sites of molecules in the extracellular milieu (e. g., albumin and histidine) and on the cell surface. Only after saturation, Co2+ is actively uptaken. CoNP, instead, are predicted to be internalised by endocytosis. Dissolution of Co particles allows the formation of Co compounds (CoNP-rel), whose mechanism of cellular internalisation is unknown. Co uptake (ranking CoMP>CoNP>Co2+) reached maximum at 4 h. Once inside the cell, CoNP spread into the cytosol and organelles. Consequently, massive amounts of Co ions and CoNP-rel can reach subcellular compartments normally unexposed to Co2+. This could explain the fact that the nuclear and mitochondrial Co concentrations resulted significantly higher than those obtained with Co2+.
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