期刊
NANOTOXICOLOGY
卷 5, 期 2, 页码 269-281出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2010.536268
关键词
Nanoparticles; nanomedicine; nanotoxicology; particle toxicology
资金
- Swedish Research Council for Environment, Agricultural Sciences, and Spatial Planning (FORMAS)
- AForsk (Angpanneforeningen's Foundation for Research and Development)
- Environmental Cancer Risk, Nutrition, and Individual Susceptibility (ECNIS)
- European Union [513943]
Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.
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