期刊
NANOTECHNOLOGY
卷 19, 期 41, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/0957-4484/19/41/415605
关键词
-
资金
- National University of Singapore [ARF R-279-000-197-112/133, YIA R-279-000234-123]
- Singapore Ministry of Education [R-279-000255-112]
Novel methods for rapid, sensitive and low-cost biomolecule detection have attracted particular interest because of their wide use in medical diagnostics, food inspection and biomedical research applications. In this work, we report a simple and efficient silica nanoparticle (NP)-based fluorescent assay for ATP detection. It takes advantage of the washing and separation properties of NPs and the structure-switch property of DNA aptamers, resulting in fluorescence change of the supernatant in the presence of targets. A linear response for ATP detection was observed from 0 to 6 mM with a detection limit of similar to 34 mu M. This detection strategy could be generalized to other aptamer-based detection systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据