期刊
NANOSCALE RESEARCH LETTERS
卷 4, 期 3, 页码 204-209出版社
SPRINGEROPEN
DOI: 10.1007/s11671-008-9226-1
关键词
Magnetic nanoparticles; Culture medium; Protein; Surface property; Cellular uptake
资金
- National Natural Science Foundation of China [60571031, 60501009, 90406023]
- National Basic Research Program of China [2006CB933200, 2006CB705600]
- Open Project Foundation of Laboratory of Solid State Microstructures of Nanjing University
Monodisperse magnetic nanoparticles (MNPs) were synthesized by thermal decomposition of iron-oleate and functionalized with silanes bearing various functional groups such as amino group (NH2), short-chain poly(ethylene glycol) (PEG), and carboxylic group (COOH). Then, silanes-functionalized magnetic nanoparticles (silanes-MNPs) were incubated in cell culture medium plus fetal calf serum to investigate the effects of proteins from culture medium on surface property of MNPs. Zeta potential measurements showed that although surface charges of silanes-MNPs were different, they exhibited negative charges at neutral pH and approximate isoelectric points after they were incubated in cell culture medium. The reason was that silanes-MNPs could easily adsorb proteins from culture medium via non-covalent binding, resulting in the formation of protein-silanes-MNPs conjugates. Moreover, silanes-MNPs with various functional groups had different adsorption capacity to proteins, as confirmed by Coomassie blue fast staining method. The in vitro cell experiments showed that protein-silanes-MNPs had higher cellular uptake by cancer cells than silanes-MNPs.
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