期刊
NANOSCALE
卷 10, 期 10, 页码 4913-4920出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nr09342j
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资金
- Natural Science Foundation of China [21405010, 21375013, 21675019, 21235001]
- Fundamental Research Funds for the Central Universities [N160504008, N141008001]
As important biomarkers, glycoprotein sensing is frequently facilitated by boronic acid binding with its cisdiols. However, boronic acid based sensors suffer from drawbacks of alkali restriction and/or sensitivity limitation. Herein, we report boronic acid decorated g-C3N4 nanosheets (B-g-CN) with a Wulff-type boronic acid feature, which selectively bind glycoprotein under physiological conditions. Meanwhile, the binding causes significant enhancement of the B-g-CN nanosheet fluorescence, providing the basis for glycoprotein sensing. With IgG as a model, a detection limit (LOD) of 2.2 nM (3 sigma/s, n = 11) was obtained within a linear range of 6.7-67 nM. The LOD was further improved to 52 pM subject to enrichment of the nanosheets, which well enables IgG assay in human urine samples. Moreover, it was successful in imaging endogenous and exogenous glycoproteins in living cells.
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