期刊
NANOSCALE
卷 6, 期 19, 页码 11372-11379出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4nr03195d
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资金
- NHGRI NIH HHS [R56 HG009338] Funding Source: Medline
- NIGMS NIH HHS [R01 GM079613, R01 GM114204] Funding Source: Medline
Nanopores provide a unique single-molecule platform for genetic and epigenetic detection. The target nucleic acids can be accurately analyzed by characterizing their specific electric fingerprints or signatures in the nanopore. Here we report a series of novel nanopore signatures generated by target nucleic acids that are hybridized with a probe. A length-tunable overhang appended to the probe functions as a sensor to specifically modulate the nanopore current profile. The resulting signatures can reveal multiple mechanisms for the orientational trapping, unzipping, escaping and translocation of nucleic acids in the nanopore. This universal approach can be used to program various molecular movement pathways, elucidate their kinetics, and enhance the sensitivity and specificity of the nanopore sensor for nucleic acid detection.
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