期刊
NANOSCALE
卷 5, 期 3, 页码 1121-1126出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2nr33107a
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资金
- National Natural Science Foundation of China [21275062, 21075047]
- self-determined research funds of CCNU from the colleges' basic research and operation of MOE [CCNU11C01002]
- CounterACT Program, Office of the Director, National Institutes of Health (OD)
- National Institute of Neurological Disorders and Stroke (NINDS) [U01 NS058161-01]
- US-DOE [DE-AC05-76RL01830]
A nanocomposite of gold nanoparticles (AuNPs) decorating a magnetic Fe3O4 core was synthesized using cysteamine (SH-NH2) as linker, and characterized by TEM, XPS, UV and electrochemistry. Then a hydrolase biosensor, based on self-assembly of methyl parathion hydrolase (MPH) on the Fe3O4@Au nanocomposite, was developed for sensitive and selective detection of the organophosphorus pesticide (OP) methyl parathion. The magnetic nanocomposite provides an easy way to construct the enzyme biosensor by simply exerting an external magnetic field, and also provides a simple way to renew the electrode surface by removing the magnet. Unlike inhibition-based enzyme biosensors, the hydrolase is not poisoned by OPs and thus is reusable for continuous measurement. AuNPs not only provide a large surface area, high loading efficiency and fast electron transfer, but also stabilize the enzyme through electrostatic interactions. The MPH biosensor shows rapid response and high selectivity for detection of methyl parathion, with a linear range from 0.5 to 1000 ng mL(-1) and a detection limit of 0.1 ng mL(-1). It also shows acceptable reproducibility and stability. The simplicity and ease of operation of the proposed method has great potential for on-site detection of P-S containing pesticides and provides a promising strategy to construct a robust biosensor.
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