Flash photo stimulation of human neural stem cells on graphene/TiO2 heterojunction for differentiation into neurons

For the application of human neural stem cells (hNSCs) in neural regeneration and brain repair, it is necessary to stimulate hNSC differentiation towards neurons rather than glia. Due to the unique properties of graphene in stem cell differentiation, here we introduce reduced graphene oxide (rGO)/TiO2 heterojunction film as a biocompatible flash photo stimulator for effective differentiation of hNSCs into neurons. Using the stimulation, the number of cell nuclei on rGO/TiO2 increased by a factor of similar to 1.5, while on GO/TiO2 and TiO2 it increased only similar to 48 and 24%, respectively. Moreover, under optimum conditions of flash photo stimulation (10 mW cm(-2) flash intensity and 15.0 mM ascorbic acid in cell culture medium) not only did the number of cell nuclei and neurons differentiated on rGO/TiO2 significantly increase (by factors of similar to 2.5 and 3.6), but also the number of glial cells decreased (by a factor of similar to 0.28). This resulted in a similar to 23-fold increase in the neural to glial cell ratio. Such highly accelerated differentiation was assigned to electron injection from the photoexcited TiO2 into the cells on the rGO through Ti-C and Ti-O-C bonds. The role of ascorbic acid, as a scavenger of the photoexcited holes, in flash photo stimulation was studied at various concentrations and flash intensities.