期刊
NANOSCALE
卷 5, 期 7, 页码 2756-2764出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3nr33403a
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- National Science Council of Taiwan [NSC 101-2113-M-002-002-MY3]
- National Health Research Institutes (Taiwan) [NHRI-EX100-10047NI]
We have developed aptamer (Apt)-conjugated gold nanoparticles (Apt-Au NPs, 13 nm in diameter) as highly effective inhibitors for human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). Two Apts, RT1t49 (Apt(pol)) and ODN 93 (Apt(RH)), which recognize the polymerase and RNase H regions of HIV-1 RT, are used to conjugate Au NPs to prepare Apt(pol)-Au NPs and Apt(RH)-Au NPs, respectively. In addition to DNA sequence, the surface density of the aptamers on Au NPs (nApt-Au NPs; n is the number of aptamer molecules on each Au NP) and the linker length number (T-m; m is the base number of the deoxythymidine linker) between the aptamer and Au NPs play important roles in determining their inhibition activity. A HIV-lentiviral vector-based antiviral assay has been applied to determine the inhibitory effect of aptamers or Apt-Au NPs on the early stages of their replication cycle. The nucleasestable G-quadruplex structure of 40Apt(RH)-T-45-Au NPs shows inhibitory efficiency in the retroviral replication cycle with a decreasing infectivity (40.2%).
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