期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 206, 期 -, 页码 268-275出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.09.052
关键词
Sulfite; Fluorescent probe; Ratiometric; Benzopyrylium; Coumarin
资金
- National Natural Science Foundation of China [21475105, 21275117, 21375105]
- Science & Technology Department [2012JM2004]
- Education Department of Shaanxi Province of China [12JK0518]
A ratiometric fluorescent probe, 2-(7-diethylamino-2-oxo-2H-1-benzopyran-3-yl)-7-hydroxyl-1-benzopyrylium (1), has been developed for sulfite sensing. The method employs the nucleophilic addition of sulfite to the electrically positive benzopyrylium moiety of 1 to block the pi-conjugated system of the whole molecule, which in turn results in significant blue shifts in the absorption and emission spectra of the sensing system. The fluorescence intensity ratio at 485 and 640 nm (I-485/I-640) increases linearly with sulfite concentration in the range of 0.05-10 mu M. The proposed probe shows excellent selectivity toward sulfite over other common anions and biothiols. The bioimaging experiment demonstrates the potential of probe 1 for the ratiometric fluorescent imaging of sulfite in living cells. (C) 2014 Elsevier B.V. All rights reserved.
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