期刊
NANOSCALE
卷 3, 期 7, 页码 2971-2977出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1nr10240k
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- Council of Scientific & Industrial Research (CSIR)
- Department of Science and Technology (DST), India
- DST [DST/TSG/ME/2008/18]
Urease (Urs) and glutamate dehydrogenase (GLDH) have been covalently co-immobilized onto a self-assembled monolayer (SAM) comprising of 10-carboxy-1-decanthiol (CDT) via EDC-NHS chemistry deposited onto one of the two patterned gold (Au) electrodes for estimation of urea using poly(dimethylsiloxane) based microfluidic channels (2 cm x 200 mu m x 200 mu m). The CDT/Au and Urs-GLDH/CDT/Au electrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, contact angle (CA), atomic force microscopy (AFM) and electrochemical cyclic voltammetry (CV) techniques. The electrochemical response measurement of a Urs-GLDH/CDT/Au bioelectrode obtained as a function of urea concentration using CV yield linearity as 10 to 100 mg dl(-1), detection limit as 9 mg dl(-1) and high sensitivity as 7.5 mu A mM(-1) cm(-2).
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