期刊
NANOSCALE
卷 3, 期 9, 页码 3747-3754出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1nr10435g
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- Defence Research and Development Canada Centre for Security Science Chemical, Biological, Radiological/Nuclear, and Explosives Research and Technology [CRTI-03-0005RD, CRTI-06-0187TD]
- National Research Council
A silica nanoparticle-based DNA biosensor capable of detecting Bacillus anthracis bacteria through the use of unlabelled ss-oligonucleotides has been developed. The biosensor makes use of the optical changes that accompany a nanoparticle-immobilized cationic conjugated polymer (polythiophene) interacting with single-stranded vs. hybridized oligonucleotides, where a fluorescence signal appears only when hybridized DNA is present (i.e. only when the ss-oligonucleotide interacting with the polymer has hybridized with its complement). In order to enhance the sensitivity of the biosensor, two different nanoparticle architectures were developed and used to elucidate how the presence of neighboring fluorophores on the nanoparticle surface affects Forster-resonant energy transfer (FRET) between the polythiophene/oligonucleotide complex (FRET donor) and the fluorophores (FRET acceptors). We demonstrate that the silica nanoparticle-based FRET platform lowers the limit of detection at least 10-fold in comparison to the polythiophene itself, and allows the detection of similar to 2 x 10(-12) moles of ss-oligonucleotide in a 100 mu L sample with a standard fluorimeter (i.e. has a limit of detection of similar to 2 nM ssDNA). Such nanoparticle-based biosensor platforms are beneficial because of the robustness and stability inherent to their covalent assembly and they provide a valuable new tool that may allow for the sensitive, label-free detection (the target DNA that produces the fluorescence signal is unlabelled) without the use of polymerase chain reaction.
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