期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 216, 期 -, 页码 255-262出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.04.014
关键词
Open bipolar electrode; Electrochemiluminescence; Paper-based Microfluidics; Visual detection; Wax-screen-printing; Small-batch manufacturing
资金
- National Natural Science Foundation of China [61072030]
- project of Guangzhou science and technology plan [2014J4100030]
A novel paper-based open bipolar electrode-electrochemiluminescence (P-o-BPE-ECL) sensing has been developed for visual detection. Wax-screen-printing was employed to make microfluidic channels on filter paper, and the carbon ink-based BPE and driving electrodes were screen-printed on paper. An inexpensive CCD camera was used as a detector for imaging sensing. In this study, two P-o-BPE-ECL imaging sensing platforms were demonstrated. For the first one, a hydrophilic paper channel was used to host a single band-shaped BPE. Under optimal conditions, this P-o-BPE-ECL platform successfully fulfilled the imaging sensing of tri-n-propylamine (TPA) with a linear range from 10 to 1000 mu M with a detection limit of 8.7 mu M. In addition, the determination of H2O2 using this platform could be performed in the linear range of 50 mu M to 5000 mu M with a detection limit of 46.6 mu M. For the second platform, an open BPE array configuration has been proposed for P-o-BPE-ECL sensing, where eleven BPEs were stridden across the two legs of the U-shaped paper channel. On this prototype, a simple screening application has been performed. These results show that the proposed strategy offers great promise in providing a simple, low-cost, rapid and environment-friendly solution for biochemical applications. (C) 2015 Elsevier B.V. All rights reserved.
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