期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 10, 期 8, 页码 1701-1709出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2014.05.014
关键词
Two-photon imaging; Quantum dots; Single-domain antibodies; Diagnostic nanoprobes; Immunohistochemistry
资金
- European Commission [NMP-2009-4.0-3-246479 NAMDIATREAM]
- Ministry of Higher Education and Science of the Russian Federation [11.G34.31.0050]
- Champagne-Ardenne region
- DRRT Champagne-Ardenne
- FEDER
- Russian Science Foundation
An ideal multiphoton fluorescent nanoprobe should combine a nanocrystal with the largest possible two-photon absorption cross section (TPACS) and the smallest highly specific recognition molecules bound in an oriented manner. CdSe/ZnS quantum dots (QDs) conjugated to 13-kDa single-domain antibodies (sdAbs) derived from camelid IgG or streptavidin have been used as efficient two-photon excitation (TPE) probes for carcinoembryonic antigen (CEA) imaging on normal human appendix and colon carcinoma tissue. The TPACS for some conjugates was higher than 49,000 GM (Goeppert-Mayer units), considerably exceeding that of organic dyes being close to the theoretical value of 50,000 GM calculated for CdSe QDs. The ratio of sdAb-QD emission to the autofluorescence for 800 nm TPE was 40 times higher than that for 457.9 nm one-photon excitation. TPE ensures a clear discrimination of CEA-overexpressing tumor areas from normal tissue. Oriented sdAb-QD conjugates are bright specific labels for detecting low concentrations of antigens using multiphoton microscopy. From the Clinical Editor: This study demonstrates carcinoembryonic antigen imaging on normal human appendix and colon carcinoma tissue utilizing CdSe/ZnS quantum dots conjugated to streptavidin or to 13-kDa single-domain antibodies as efficient two-photon excitation probes. (C) 2014 Elsevier Inc. All rights reserved.
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