期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 9, 期 6, 页码 737-741出版社
ELSEVIER
DOI: 10.1016/j.nano.2013.05.014
关键词
Neural stem cell; Neurosphere; Magnetic nanoparticle; Transfection; Nonviral
资金
- British Biotechnology and Biological Sciences Research Council (BBSRC)
- Engineering and Physical Sciences Research Council (EPSRC) via the Doctoral Training Centre in Regenerative Medicine
- BBSRC [BB/J017590/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J017590/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1119377] Funding Source: researchfish
Safe genetic modification of neural stem cell (NSC) transplant populations is a key goal for regenerative neurology. We describe a technically simple and safe method to increase transfection in NSCs propagated in the neurosphere (suspension culture) model, using magnetic nanoparticles deployed with applied oscillating magnetic fields ('magnetofection technology'). We show that transfection efficiency was enhanced over two-fold by oscillating magnetic fields (frequency = 4 Hz). The protocols had no effect on cell viability, cell number, stem cell marker expression and differentiation profiles of 'magnetofected' cultures, highlighting the safety of the technique. As far as we are aware, this is the first successful application of magnetofection technology to suspension cultures of neural cells. The procedures described offer a means to augment the therapeutic potential of NSCs propagated as neurospheres - a culture model of high clinical translational relevance - by safe genetic manipulation, with further potential for incorporation into 'magneto-multifection' (repeat transfection) protocols. From the Clinical Editor: This team of investigators describe a simple and safe method to increase transfection in neural stem cells using magnetic nanoparticles deployed with oscillating magnetic fields, demonstrating a greater than two-fold transfection efficiency increase by applying low frequency magnetic oscillation. (c) 2013 Elsevier Inc. All rights reserved.
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